| 292 | 1 | 31 |
| 下载次数 | 被引频次 | 阅读次数 |
植物多糖具有改善心肺功能、保护肝脏、抵抗病毒和肿瘤、消除炎症、调节肠道菌群、降低血糖和血脂、增强机体免疫力等生物学功能。与合成聚合物相比，植物多糖无毒、可生物降解、具有良好的生物相容性和低廉的价格，受到广泛关注。猪精液在冻存时会伴随腺嘌呤核苷三磷酸消耗和精子老化，并产生活性氧等有害副产物，影响人工授精后母猪受胎率。为此，生产中常向猪精液稀释液中添加黄芪多糖、枸杞多糖、红景天多糖、海带多糖、丹参多糖等植物多糖，以减缓有害副产物的产生，提高精子抗低温打击能力，减缓氧化应激，改善冻存精液品质，提高母猪受孕率。本文就植物多糖改善精液冻存效果的作用机理及应用进行综述，以期为畜牧业高质量发展提供科学参考。
Abstract：Plant polysaccharides have biological functions such as improving cardiovascular function, protecting the liver,resisting viruses and tumors, eliminating inflammation, regulating gut microbiota, lowering blood sugar and lipids, and enhancing the body's immunity. Compared with synthetic polymers, it has attracted widespread attention due to its nontoxic, biodegradable, good biocompatibility, and low cost. Cryopreservation of pig sperm is accompanied by the consumption of adenosine triphosphate(ATP) and sperm aging, as well as the production of harmful products such as excessive reactive oxygen species(ROS), resulting in unsatisfactory fertilization rates for artificially inseminated sows. Therefore, in practice,plant polysaccharides such as Astragalus polysaccharides, goji berry polysaccharides, Rhodiola polysaccharides, kelp polysaccharides, and Salvia miltiorrhiza polysaccharides are often added to pig sperm diluents to slow down the production of harmful products, improve sperm resistance to low-temperature shocks, and alleviate oxidative stress, thereby improving the quality of frozen sperm and increasing the pregnancy rate of sows. This article mainly provides an overview of the mechanism and application of plant polysaccharides for improving the cryopreservation efficiency of sperm in order to provide scientific reference for the high-quality development of animal husbandry.
[1]邵会敏,杨元森,陈莹,等.植物多糖的抗氧化功能及其在动物生产中的应用研究进展[J].饲料研究, 2021, 44(13):119-122.
[2]齐红欢,胡德宝,秦顺义,等.植物多糖提高猪抗病能力研究进展[J].猪业科学, 2022, 39(6):82-85.
[3] Wang Y, Ji X, Yan M, et al. Protective effect and mechanism of polysaccharide from Dictyophoraindusiata on dextran sodium sulfateinduced colitis in C57BL/6 mice[J]. Int J Biol Macromol,2019, 140:973-984.
[4]梁金强,王园园,胡明华,等.复合多糖对CTX致免疫低下小鼠的免疫增强作用[J].中药材, 2017, 40(4):953-956.
[5]边亮,陈华国,周欣.植物多糖的抗肿瘤活性研究进展[J].食品科学, 2020, 41(7):275-282.
[6] Zhao D, Zhang Y, Yuan Z, et al. Study of the protective effect of Schizandrachinensis polysaccharide on oxidative damage of mouse spermatozoa in vitro[C]. The 2014 International Conference on Frontiers of Advanced Materials and Engineering Technology(FAMET 2014), Hongkong:Trans Tech Publications, 2014.
[7]吴琳.三种抗氧化剂对猪精子保存效果的研究[D].杨凌:西北农林科技大学, 2019.
[8]谢言射,徐志谦,蔡更元,等.猪精液冷冻保存技术研究进展[J].家畜生态学报, 2020, 41(10):78-83.
[9]张群,王骞,郭铭晖,等.猪精子冷冻损伤与保护剂的研究进展[J].猪业科学, 2021, 38(2):106-108.
[10] Xu D, Wang C H, Zhuo Z, et al. Extraction, purification and antioxidant activity of polysaccharide from cold pressedoil cake of ‘Tengjiao’ seed[J]. Int J Biol Macromol, 2020, 163:508-518.
[11]慕雪,刘垠泽,吴恒梅.白心火龙果果皮多糖提取及抗氧化研究[J].中国野生植物资源, 2023, 42(3):21-25, 37.
[12]杨宗芬,王敏,王贞利,等.冰菜多糖提取物的抗氧化性及抑菌活性研究[J].天津农学院学报, 2023, 30(1):1-5.
[13] Pezo F, Yeste M, Zambrano F, et al. Antioxidants and their effect on the oxidative/nitrosative stress of frozen-thawed boar sperm[J]. Cryobiology, 2021, 98:5-11.
[14] Zhang B, Wang Y, Wu C, et al. Freeze-thawing impairs the motility, plasma membrane integrity and mitochondria function of boar spermatozoa through generating excessive ROS[J].BMC Vet Res, 2021, 17(1):127.
[15]许兰仙,马文平.多糖抗氧化研究进展[J].食品界,2017(9):48-49.
[16] Varela E, Rojas M, Restrepo G. Membrane stability and mitochondrial activity of bovine sperm frozen with low-density lipoproteins and trehalose[J]. Reprod Domest Anim, 2020,55(2):146-153.
[17] Tuo X L, Deng Z J, Huang G C, et al. Astragalus polysaccharide attenuates over exercise-induce myocardial injury via activating AMPK signaling pathway to suppress inflammation and oxidative stress[J]. An Acad Bras Cienc, 2021, 94(1):e20210314.
[18] Yin Y, Lu L, Wang D T, et al. Astragalus polysaccharide inhibits autophagy and apoptosis from peroxide-induced injury in C2C12 myoblasts[J]. Cell Bio chem Biophys, 2015, 73(2):433-439.
[19]李珣,杨尚雪,王晓晔,等.黄芪多糖对冷冻前后猪精液中过氧化氢酶活性及其mRNA表达的影响[J].中国兽医科学,2015, 45(4):435-440.
[20] Weng X G, Cai M M, Zhang Y T, et al. Effect of astragalus polysaccharide addition to thawed boar sperm on in vitro fertilization and embryo development[J]. Theriogenology, 2018,121:21-26.
[21]苟春阳,商昊,窦浩荡,等.黄芩多糖对长白猪精液冷冻保存效果的研究[J].中国畜牧杂志, 2024, 60(1):274-278.
[22] Hui H, Gao W. Structure characterization, antioxidant and hypoglycemic activity of an arabinogalactoglucan from Scutellaria baicalensis Georgi[J]. Int J Biol Macromol, 2022, 207:346-357.
[23] Zhang L L, Gu J X, Chen Y J, et al. A study on four antioxidation effects of Lyciumbarbarum polysaccharides in vitro[J].Afr J Tradit Complem, 2013, 10(6):494-498.
[24] Cheng J, Zhou Z W, Sheng H P, et al. An evidence-based update on the pharmacological activities and possible molecular targets of Lycium barbarum polysaccharides[J]. Drug Des Dev Ther,2015, 9:33-78.
[25]颜贝,王娟,周岳,等.枸杞多糖对冻存精子线粒体保护作用的研究[J].时珍国医国药, 2020, 31(9):2110-2113.
[26]何涛,李青旺,王斌.枸杞多糖对猪精液冷冻效果影响的研究[J].陕西农业科学, 2009(4):3-5.
[27]王硕,赵家辉,邹一诺,等.枸杞多糖对17℃负压环境下猪精子保存质量的影响[J].中国畜牧杂志, 2020, 56(5):95-99.
[28]冯妮,陈志英,谢运法,等.枸杞多糖对获能新鲜和冻后猪精子抗氧化能力的影响[J].畜牧与兽医, 2021, 53(9):16-20.
[29] Li M H, Tang C F, Ouyang J Q. Influence of salidroside from Rhodiola Sachalinensis A. Bor on some related indexes of free radical and energy metabolism after exercise in mice[J]. Chin J Appl Physiol, 2012, 28(28):53-56.
[30]贺佳林,胡瑞成,戴爱国.红景天抗氧化及对细胞因子作用的研究进展[J].临床与病理杂志, 2015, 35(2):272-277.
[31] Kanupriya, Dipti P, Sharma S K, et al. Flavonoids protect U-937 macrophages against tert-butylhydroperoxide induced oxidative injury[J]. Food Chem Toxicol, 2006, 44(7):1024-1030.
[32] Wang X B, Hou Y, Li Q Y, et al. Rhodiolacrenulata attenuates apoptosis and mitochondrial energy metabolism disorder in rats with hypobaric hypoxia-induced brain injury by regulating the HIF-1 α/microRNA 210/ISCU1/2(COX10)signaling pathway[J]. J Ethnopharmacol, 2019, 241:111801.
[33]任子利,宋天增,张健,等.红景天多糖对藏猪精液冻后品质及精子基因组DNA甲基化的影响[J].中国兽医学报,2017, 37(7):1385-1388, 1393.
[34] Yang S M, Wang T, Wen D G, et al. Protective effect of Rhodiolarosea polysaccharides on cryopreserved boar sperm[J].Carbohydr Polym, 2016, 135:44-47.
[35]韩晓,戴建军,张树山,等.纳米化红景天多糖对猪精液冷冻效果的影响[J].中国兽医学报, 2013, 33(12):1937-1942.
[36]江中良. LDL和多糖在猪精液冷冻保存中的作用研究[D].杨凌:西北农林科技大学, 2010.
[37] Woelders H, Matthijs A, Engel B. Effects of trehalose and sucrose, osmolality of the freezing medium, and cooling rate on viability and intactness of bull sperm after freezing and thawing[J]. Cryobiology, 1997, 35(2):93-105.
[38] Aisen E G, Medina V H, Venturino A. Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations[J]. Theriogenology, 2002, 57(7):1801-1808.
[39] Hou Y, Wang J, Jin W H, et al. Degradation of Laminaria japonica fucoidan by hydrogen peroxide and antioxidant activities of the degradation products of different molecular weights[J].Carbohydr Polym, 2012, 87(1):153-159.
[40] Yin D F, Sun X J, Li N, et al. Structural properties and antioxidant activity of polysaccharides extracted from Laminaria japonica using various methods[J]. Process Biochem, 2021,111(P2):201-209.
[41]何涛,李军.海带多糖对猪精液冷冻效果影响的研究[J].畜牧与饲料科学, 2013, 34(1):23-26.
[42]潘巧莲,李青旺,周圣贤,等.海带多糖和红景天多糖对猪精子冷冻效果的影响[J].黑龙江畜牧兽医, 2009(19):33-34.
[43] Hu J H, Sun X Z, Li Q W, et al. The effect of Laminaria japonic polysaccharide on sperm characteristics and biochemical parameters in cryopreserved boar sperm[J]. Anim Reprod Sci, 2013,139(1-4):95-100.
[44] Geng Y M, Lu C Y, Jin G Z, et al. Study on the mechanism of Salvia miltiorrhiza polysaccharides in relieving liver injury of broilers induced by florfenicol[J]. Environ Sci Pollut Res, 2022,29(3):3372-3385.
[45]祁小妮,马晓红,李振亮,等.响应曲面优化丹参多糖提取工艺及抗氧化活性分析[J].南方农业学报, 2016, 47(11):1926-1931.
[46] Jiang Y Y, Wang L, Zhang L, et al. Optimization of extraction and antioxidant activity of polysaccharides from Salvia miltiorrhiza Bunge residue[J]. Int J Biol Macromol, 2015, 79:533-541.
[47] Shen T, Jiang Z L, Liu H, et al. Effect of Salvia miltiorrhiza polysaccharides on boar spermatozoa during freezing-thawing[J]. Anim Reprod Sci, 2015, 159:25-30.
[48]沈涛.丹参多糖和α-硫辛酸对猪精液冷冻保存效果的影响研究[D].杨凌:西北农林科技大学, 2015.
[49]张婷.四种糖类对猪精液冷冻保存效果的研究[D].杨凌:西北农林科技大学, 2007.
[50]李青旺,江中良,胡建宏,等.多糖在猪精液冷冻稀释液的作用研究[A].中国畜牧兽医学会动物繁殖学分会第十五届学术研讨会论文集（下册）[C].天津:中国畜牧兽医学会,2010:5.
[51]曹华琳.红景天和益母草多糖对牛猪精液冷冻保存效果的研究[D].杨凌:西北农林科技大学，2014.
[52]周岳,颜贝,田稼,等.淫羊藿多糖对弱精症精子冷冻保存效果的影响[J].天然产物研究与开发, 2021, 33(6):1013-1019.
[53]李军伟.淫羊藿多糖对猪精液冷冻保存的影响[D].南京:南京农业大学, 2013.
[54]刘颖.茯苓多糖、黄芪多糖和银杏叶黄酮对猪精子冷冻保存效果的研究[D].杨凌:西北农林科技大学, 2015.
[55]罗芬芳,胡善辉,万培伟,等.甘草多糖对猪精液低温保存的影响[J].江西畜牧兽医杂志, 2021(6):17-19.
[56]何涛.绿原酸、木犀草素和黄精多糖对猪精液冷冻保存效果的研究[D].兰州:甘肃农业大学, 2022.
[57]孙艳青.葡萄籽原花青素、褪黑激素和大枣多糖对猪精子冷冻保存效果的影响[D].杨凌:西北农林科技大学, 2016.
基本信息:
DOI：10.19556/j.0258-7033.20231202-01
中图分类号:S828.3
引用信息:
[1]董小英,唐雨辰,唐胜球.植物多糖改善猪精液冻存效果的应用研究进展[J].中国畜牧杂志,2024,60(12):80-85.DOI:10.19556/j.0258-7033.20231202-01.
基金信息:
广东省自然科学基金（2023A1515010104、2024A1515013150）; 广东省高校人工智能和服务乡村振兴重点领域专项（2019KZDZX2007）; 韶关市科技支撑乡村振兴项目（230615148039793）; 广东省高校“双带头人”教师党支部书记“广东行”专项行动（粤教工委组函[2024]12号）